Dear Rahul Sir,
No that will not happen as in common practice you don't consider eligibility of ESIC on commission or incentive but you have to pay the contribution on actual gross.
Example: If a person have gross salary 20000/- in normal course. If in month he gets commission of say 10000 then the gross salary calculates to be 30000/- in such case ESIC must be deducted but, on 30000/-
just because employee got commission in one month doesn't mean that you should opt that employee out of ESIC.